研究業績集

投稿論文2015年

タイトル

Noncompetitive immunoassay detection system for haptens on the basis of antimetatype antibodies

著者

Kazuya Omi, Tsuyoshi Ando, Takuya Sakyu, Takashi Shirakawa, Yoshiaki Uchida, Asako Oka, Nobuyuki Ise, Katsumi Aoyagi and Katsutoshi Goishi

雑誌名

Clin Chem. 2015 Apr;61(4):627-35.

内容

Small molecules are generally measured by competitive immunoassays, which are theoretically inferior to sandwich immunoassays in terms of sensitivity and specificity. We generated antimetatype antibodies against corresponding hapten-antibody immunocomplexes using an ex vivo antibody development system, the Autonomously Diversifying Library (ADLib) system. Sandwich immunoassays for Estradiol (E2) and 25-hydroxyvitamin D [25(OH)D] were developed by use of the antimetatype antibodies. These assays exhibited sensitivity high enough for clinical use, while their cross-reactivity to immunoreactive derivatives of their target molecules was effectively improved compared with the competitive assays. In particular, the measurement obtained with the sandwich CLEIA for 25(OH)D well correlated with the measurements by LC-MS/MS and a commercially available 125I RIA (r = 0.99). Our method represents a practical approach for routine assays of hapten measurement.

タイトル

ルミパルスG1200を用いた尿中L型脂肪酸結合蛋白測定試薬(ルミパルス L-FABP)の基礎的検討

著者

富田健一郎1)、寺尾梓、桑原麻樹1)、岡村智郷、阿部直也1)
1)日本赤十字社医療センター検査部

雑誌名

医学と薬学、2015;72:1389-1395